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KMID : 1123920030170051335
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Korean Journal of Oriental Physiology and Pathology
2003 Volume.17 No. 5 p.1335 ~ p.1338
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Vasodilation of BCT is Associated with Inhibition of PKC Translocation and LC20 Phosphorylation
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Kwon Oh-Kui
Shin Dong-Hoon
Kim Gil-Whon
Shin Heung-Mook
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Abstract
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We have previously reported that the vasodilatory effect of BanhabackchuIChunma-tang(半夏白朮天麻湯; BCT), a herbal formula, and its mechanism might be associated with at least in part NO pathway. In the present study, we studied the influence of BanhabackchulChenuma-tang (BCT) on the phosphorylation of LC20, in parallel, the distribution of α-protein kinase C(PKCα) by phenylephrine was monitored using laser scan confocal immunofluorescent microscopy in freshly isolated ferret portal vein smooth muscle living single cells. Phenylephrine stimulation induced LC20 phosphorylation and translocation of PKCα. However, BCT dephosphorylated LC20 phosphorylation and inhibited the translocation of PKCα. Our results demonstrate that the mechanism of relaxant effect of BanhabackchulChunma-tang inhibition is associated with inhibition of PKCα activation and LC20 phosphorylation.
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KEYWORD
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BanhabackchulChunma-tang(半夏白朮天麻湯), ferret portal vein, PKCα, LC20, vasodilation
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